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首页> 外文OA文献 >Expression of Genes Involved in Anthocyanin Biosynthesis in Relation to Anthocyanin, Proanthocyanidin, and Flavonol Levels during Bilberry Fruit Development1
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Expression of Genes Involved in Anthocyanin Biosynthesis in Relation to Anthocyanin, Proanthocyanidin, and Flavonol Levels during Bilberry Fruit Development1

机译：越橘果实发育过程中花色苷生物合成相关基因的表达与花色苷，原花色素和黄酮水平的关系1

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The production of anthocyanins in fruit tissues is highly controlled at the developmental level. We have studied the expression of flavonoid biosynthesis genes during the development of bilberry (Vaccinium myrtillus) fruit in relation to the accumulation of anthocyanins, proanthocyanidins, and flavonols in wild berries and in color mutants of bilberry. The cDNA fragments of five genes from the flavonoid pathway, phenylalanine ammonia-lyase, chalcone synthase, flavanone 3-hydroxylase, dihydroflavonol 4-reductase, and anthocyanidin synthase, were isolated from bilberry using the polymerase chain reaction technique, sequenced, and labeled with a digoxigenin-dUTP label. These homologous probes were used for determining the expression of the flavonoid pathway genes in bilberries. The contents of anthocyanins, proanthocyanidins, and flavonols in ripening bilberries were analyzed with high-performance liquid chromatography-diode array detector and were identified using a mass spectrometry interface. Our results demonstrate a correlation between anthocyanin accumulation and expression of the flavonoid pathway genes during the ripening of berries. At the early stages of berry development, procyanidins and quercetin were the major flavonoids, but the levels decreased dramatically during the progress of ripening. During the later stages of ripening, the content of anthocyanins increased strongly and they were the major flavonoids in the ripe berry. The expression of flavonoid pathway genes in the color mutants of bilberry was reduced. A connection between flavonol and anthocyanin synthesis in bilberry was detected in this study and also in previous data collected from flavonol and anthocyanin analyses from other fruits. In accordance with this, models for the connection between flavonol and anthocyanin syntheses in fruit tissues are presented.
机译：在发育水平上高度控制水果组织中的花色苷生成。我们已经研究了越橘（越桔越桔）果实发育过程中黄酮类生物合成基因的表达与花青素，原花色素和黄酮醇在野生浆果和越橘颜色突变体中的积累有关。使用聚合酶链反应技术从越桔中分离出黄酮途径，苯丙氨酸氨解酶，查尔酮合酶，黄烷酮3-羟化酶，二氢黄酮醇4-还原酶和花色素苷合酶这五个基因的cDNA片段，进行测序，并用a标记。地高辛配基-dUTP标签。这些同源探针用于确定越桔中类黄酮途径基因的表达。用高效液相色谱-二极管阵列检测器分析成熟越桔中花青素，原花青素和黄酮醇的含量，并使用质谱接口进行鉴定。我们的结果证明了浆果成熟过程中花色苷积累与类黄酮途径基因表达之间的相关性。在浆果发育的早期阶段，原花青素和槲皮素是主要的类黄酮，但在成熟过程中其含量急剧下降。在成熟的后期，花青素的含量强烈增加，它们是成熟浆果中的主要类黄酮。减少了越橘颜色突变体中黄酮途径基因的表达。在这项研究中以及在从黄酮醇和其他水果的花青素分析中收集的先前数据中，检测到了越桔中黄酮醇与花青素合成之间的联系。据此，提出了在水果组织中黄酮醇与花青素合成之间的联系模型。

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